Polypeptides, and preparation and DNA encoding thereof
US5965725A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 3, 1998 |
| Grant date | Oct 12, 1999 |
| Priority date | — |
| Expiry date | Feb 3, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/857
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to polypeptide having a primary structure of amino acid sequence shown by Sequence List Sequence No. 1 and DNA encoding for the polypeptide. The polypeptide is obtainable by following steps (1)-(3): PA1 Step (1): extracting small granule fraction of homocytes of horseshoe crab with a buffer containing protein denaturing agent and chelating agent, PA1 Step (2): subjecting said extract to reverse phase high performance liquid chromatography, PA1 Step (3): eluting by concentration gradient elution with a hydrophobic organic solvent. Also, the polypeptide is produced by chemical synthesis. The polypeptide has similar chemical structure to defensin and is useful as gargles, disinfectants, antiseptics or antimicrobials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.