Compositions and methods for generating red chemiluminescence
US5965736A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 9, 1998 |
| Grant date | Oct 12, 1999 |
| Priority date | — |
| Expiry date | Dec 9, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2334/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Compounds which generate red chemiluminescence on reaction with a phosphatase enzyme are provided as well as intermediates useful for their preparation. The chemiluminescent compounds comprise a luciferin ring system and an exocyclic enol phosphate group where the position on the luciferin ring system adjacent to the double bond is disubstituted. The chemiluminescent compounds are useful alone or within compositions containing a cationic aromatic compound in methods for producing chemiluminescence. The chemiluminescent reactions can be applied in assays for phosphatase enzymes and in assays employing enzyme-labeled specific binding pairs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.