System for hepatocyte cell isolation and collection
US5968356A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 29, 1999 |
| Grant date | Oct 19, 1999 |
| Priority date | — |
| Expiry date | Jan 29, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61B10/0045
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The invention resides in apparatus and methods for isolating and collecting cells from donor tissue for later use in medical procedures. The apparatus includes a sealed enclosure, at least one screening material inside the enclosure, an inlet port configured to conduct a fluid through the screening material, and an outlet port for receiving the fluid from the screening material and conducting the fluid out of the enclosure. A material to be screened is placed inside or conducted into the enclosure. Screened material is entrained in the fluid. The fluid carries bits of the material through the screening material and out of the enclosure for later separation from the fluid. Preferred embodiments include a series of screening materials of decreasing pore size, usually in the range from ten to one thousand micrometers in diameter. A particularly preferred embodiment includes three distinct screening materials having pore sizes of 400 micrometers, 280 micrometers, and 100 micrometers, listed in the order that the fluid passes through the screening materials. The invention is used in the collection and isolation of porcine hepatocytes for later use in medical procedures. The sealed enclo…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.