Injectable non-immunogenic cartilage and bone preparation
US5968556A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 2, 1996 |
| Grant date | Oct 19, 1999 |
| Priority date | — |
| Expiry date | Apr 2, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S623/919
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Ground bone or cartilage particles are demineralized by extraction with a low ionic strength buffer such as 20 mM HEPES containing a chelating agent and protease inhibitors, then extracted with an acidic solution such as 0.3 M citric acid, pH 4.0, containing protease inhibitors. The extracted material generally contains less than 2% by weight phosphate and less than 100 mM calcium. The phosphate content can be further reduced by treatment of the matrix with acid phosphatase, which removes residual organic phosphate. The material is useful in a method of treatment of vesicouretal reflux and other disorders where a bulking agent is effective is correcting the defect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.