Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof
US5968745A · kind A · utility
174Cited by
30References
33Claims
0Family size
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Key dates
| Filing date | Oct 14, 1997 |
| Grant date | Oct 19, 1999 |
| Priority date | — |
| Expiry date | Oct 14, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A polymer-electrode including (a) a substrate having a conductive working surface; and (b) a polymer layer on the conductive working surface. The polymer layer has a plurality of microfluidic reaction openings distributed throughout the layer. An oligonucleotide probe can be attached to the polymer layer and is available to capture target nucleic acid. A soluble mediator can diffuse freely and transfer electrons from the preselected base in the hybridized nucleic acid to the conductive working surface of the substrate. An electronic signal generated from the electron transfer reaction is detected and quantitated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.