Culture media containing glycerol that are pH and color stable when heat sterilized
US5968807A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 14, 1992 |
| Grant date | Oct 19, 1999 |
| Priority date | — |
| Expiry date | Feb 14, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M41/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A heat stable culture medium is prepared by substituting glycerol for carbohydrate components such as dextrose that are prone to Maillard reactions. A preferred medium contains pancreatic digest of casein, soytone, soluble starch, a phosphate buffer system, a salt, a pH indicator, and glycerol as a primary carbon source. The culture medium is pH and color stable when exposed to steam sterilization, including post-manufacture steam sterilization when used as the medium of a self-contained biological indicator. A self-contained sterility indicator device contains a flexible cylindrical tube having an opening at one end. The tube contains a submicron screen as a gas transmissive, bacteria impermeable window, an adsorbent wick bearing at one end viable microorganisms, and a frangible glass ampule containing the culture medium. The end of the wick containing microorganisms is located away from the ampule and adjacent the screen. A cap that seals the tube opening has an open position, and a closed position that seals the window and prevents media evaporation. During sterilization, the microorganisms adjacent the screen are exposed directly to sterilant. After sterilization, the cap is mo…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.