Methods of nucleic acid isolation
US5972613A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 9, 1997 |
| Grant date | Oct 26, 1999 |
| Priority date | — |
| Expiry date | Dec 9, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to new methods of RNA isolation that exploit the surprising discovery that RNA may be differentially precipitated from DNA. The subject methods result in the formation of an RNA-containing precipitate that has an RNA content at least two fold enriched with respect to DNA, as compared with the RNA to DNA ratio of the solution from which the RNA-containing precipitate is derived. The invention also includes various methods of DNA isolation that employ the selective precipitation of RNA. The degree of RNA enrichment achieved is often much greater than two fold; enrichment by a factor of ten or greater is frequently obtained. By precipitating RNA from a solution, the RNA may be collected by simple procedures such as centrifugation or filtration, thereby avoiding the need to bind the RNA to solid phase. The collected RNA precipitate may then be solubilized. One embodiment of the subject methods involves forming an RNA-containing nucleic acid composition, adding an RNA precipitate forming chaotropic salt to the nucleic acid composition in a sufficient concentration to form an RNA-containing precipitate, collecting the RNA-containing precipitate, and (optionally) sol…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.