Homogeneous immunoassays using enzyme inhibitors
US5972630A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 19, 1991 |
| Grant date | Oct 26, 1999 |
| Priority date | — |
| Expiry date | Aug 19, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/581
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.