Sulfonic acid group buffered dry analytical element for quantitative analysis of creatine kinase or its MB isozyme
US5972635A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 22, 1998 |
| Grant date | Oct 26, 1999 |
| Priority date | — |
| Expiry date | Sep 22, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/50
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A dry analytical element for quantitatively analyzing creatine kinase is composed of a support and a creatine kinase-detecting agent layer. The creatine kinase-detecting agent layer contains creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase, a buffer compound having a sulfonic acid group which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound. An analytical element for analysis of creatine kinase MB isozyme further contains in the layer an antibody which specifically inhibits activity of M sub-unit of creatine kinase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.