Fluorescence-based assays for measuring cell proliferation
US5972639A · kind A · utility
67Cited by
27References
26Claims
0Family size
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Key dates
| Filing date | Jul 24, 1997 |
| Grant date | Oct 26, 1999 |
| Priority date | — |
| Expiry date | Jul 24, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2334/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is for determining the number of cells in cell culture is provided. In practicing the method, the cells are lysed and incubated at about 18.degree. C. to 25.degree. C. at acid pH with a benzothiazole substrate for a sufficient time to generate detectable fluorescence that is proportional to cell number, and then measuring fluorescence. The method is highly sensitive and can detect small differences or changes in cell number. Thus, the method can be used in drug screening assays. An automated high throughput screening assay for identifying compounds that modulate angiogenesis using the method is also provided.
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