Chromatographic purification of cyclosporing using a high pressure carbon dioxide eluent
US5976381A · kind A · utility
9Cited by
3References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 12, 1997 |
| Grant date | Nov 2, 1999 |
| Priority date | — |
| Expiry date | Nov 12, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K7/645
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for the preparation of pure cyclosporin which is purified chromatographically from a mixture of different cyclosporin forms and other substances prepared by fermentation. The process comprises feeding a mixture containing a desired form of cyclosporin with an eluent consisting of high pressure carbon dioxide and an adjuvant, into a chromatography column being packed with particles prepared of silica, and recovering from the eluent flow coming out of the chromatography column a fraction which contains the desired cyclosporin form in adequately pure form.
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