Comparative Genomic Hybridization (CGH)

US5976790A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Daniel Pinkel · Lafayette, US
• Joe W. Gray · Livermore, US
• Anne Kallioniemi · Tampere, FI
• Olli-Pekka Kallioniemi · Tampere, FI
• Frederick Waldman · San Francisco, US
• Masaru Sakamoto · Kitaibaraki, JP

Key dates

• Filing date: Nov 27, 1995
• Grant date: Nov 2, 1999
• Expiry date: Nov 27, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/813
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all R…