DNA ligase assay
US5976806A · kind A · utility
19Cited by
0References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 27, 1998 |
| Grant date | Nov 2, 1999 |
| Priority date | — |
| Expiry date | May 27, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9015
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A quantitative and functional DNA ligase assay is disclosed. The assay involves the disruption, using restriction enzymes, of a plasmid containing a reporter gene, followed by a ligation step performed in the presence of the biological sample being assayed for DNA ligase activity. The ligation reaction products are then subjected to a coupled transcription-translation reaction, and the extent of DNA ligation is then quantified.
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