Eukaryotic cells stably expressing genes from multiple transfected episomes
US5976807A · kind A · utility
13Cited by
0References
26Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 6, 1998 |
| Grant date | Nov 2, 1999 |
| Priority date | — |
| Expiry date | Aug 6, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for producing recombinant eukaryotic cell lines expressing multiple proteins of interest. Eukaryotic host cells are transfected with (a) a first episome which contains an EBV origin of replication and a first gene encoding a protein of interest; and (b) a second episome containing an EBV origin of replication and a second gene encoding a protein of interest. Transfected cells are obtained expressing an EBNA 1 protein. The cells are grown under conditions wherein the episomes express the first and second genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.