Method of preparing a variant of a lipolytic enzyme
US5976855A · kind A · utility
60Cited by
1References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 22, 1996 |
| Grant date | Nov 2, 1999 |
| Priority date | — |
| Expiry date | Aug 22, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/913
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.