Process and apparatus for the genetic transformation of cells
US5976880A · kind A · utility
15Cited by
3References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 18, 1990 |
| Grant date | Nov 2, 1999 |
| Priority date | — |
| Expiry date | Dec 18, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In a tubular pressure chamber a drop of a DNA solution with gold particles suspended therein is atomized at the opening of a cannula by a pressure impact. The fog droplets containing the gold particles and the DNA entrained by them, are pressured by the pressure impact through a restriction at the end of the pressure chamber and thereby accelerated and focused. They subsequently traverse in free flight an evacuated specimen chamber and impact in a narrowly limited target area with a predetermined pulse, cells fixed on a holder, thereby penetrating them.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.