Tandem fluorescent protein constructs

US5981200A · kind A · utility

Assignees

• The Regents of the University of California · US
• Aurora Biosciences Corporation · US

Inventors

• Roger Y. Tsien · Village of La Jolla, US
• Roger Heim · Del Mar, US
• Andrew B. Cubitt · San Diego, US

Key dates

• Filing date: Jan 31, 1997
• Grant date: Nov 9, 1999
• Expiry date: Jan 31, 2017

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/43595
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.