Unitary sandwich enzyme immunoassay cassette, device and method of use
US5981203A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 13, 1997 |
| Grant date | Nov 9, 1999 |
| Priority date | — |
| Expiry date | Jan 13, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An enzyme sandwich immunoassay, device and cassette therefor for determining a target analyte are described. The enzyme sandwich immunoassay cassette (B) comprises a microporous membrane support (m) having coated thereon a conductive metal layer overlaid with a capture antibody layer (e). The electochemical device (A) comprises a first chamber (b) into which sample and an enzyme labelled antibody capable of specifically binding to the target analyte are placed, wherein the enzyme is capable of catalyzing the production of an electrochemically detectable product; a second chamber (a) adjoining the first chamber (b) into which a substrate for the enzyme is placed, wherein the substrate is capable of producing, when catalyzed by the enzyme, the electrochemically detectable product; and, separating the first (b) and second (a) chambers, a microporous membrane support (m) as described above, wherein the conductive metal layer (e) of the microporous membrane support (m) acts as an electrode to detect, directly or indirectly, the presence of the electrochemically detectable product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.