Process for the production of cell mass and/or fermentation products under sterile conditions
US5981260A · kind A · utility
Inventor
Key dates
| Filing date | Nov 21, 1991 |
| Grant date | Nov 9, 1999 |
| Priority date | — |
| Expiry date | Nov 21, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/813
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Process for the production of cell mass and/or fermentation products under sterile conditions, during which the fermentation mixture, at least at times, is recycled and the metabolic products of the cultivated cells, and possibly the cell mass are separated; the process comprises the following steps: PA1 charging the fermentation equipment with a sufficient amount of nutrient medium to start the required cell culture. PA1 sterilizing the equipment as well as adjustment of the required concentration of nutrient medium, PA1 inoculation of the nutrient medium with the starter culture and allowing undisturbed growth of the culture for a defined time, PA1 increasing the concentration of the nutrient medium to the specific nutrient medium concentration of the cell culture by simultaneously increasing the volume of the nutrient medium to the working volume of the fermenter and increasing the concentration of cells, PA1 transition to continuous procedure with exchange of the nutrient medium and separation of the metabolic products as well as complete or partial cell recycling, PA1 termination of the continuous procedure at a required time, harvest of the cell mass under sterile conditions,…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.