Primers for amplification of a genus specific sequence of the mycobacterium 16S rRNA gene
US5985569A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 1997 |
| Grant date | Nov 16, 1999 |
| Priority date | — |
| Expiry date | Sep 26, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are oligonucleotide primers and probes that can be used to detect species of medical interest in the genus Mycobacterium. Also disclosed are genus-specific methods and kits for detecting Mycobacterium using the disclosed oligonucleotide primers and probes. Preferably, Mycobacterium are detected by amplifying the Mycobactenium nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, Mycobacterium nucleic acids are amplified and detected by thermophilic strand displacement amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.