Patent · US Expired

Primers for amplification of a genus specific sequence of the mycobacterium 16S rRNA gene

US5985569A · kind A · utility

9Cited by
2References
25Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 26, 1997
Grant dateNov 16, 1999
Priority date
Expiry dateSep 26, 2017

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/689
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed herein are oligonucleotide primers and probes that can be used to detect species of medical interest in the genus Mycobacterium. Also disclosed are genus-specific methods and kits for detecting Mycobacterium using the disclosed oligonucleotide primers and probes. Preferably, Mycobacterium are detected by amplifying the Mycobactenium nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, Mycobacterium nucleic acids are amplified and detected by thermophilic strand displacement amplification.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.