Modified carboxypeptidase
US5985627A · kind A · utility
4Cited by
3References
28Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1997 |
| Grant date | Nov 16, 1999 |
| Priority date | — |
| Expiry date | Feb 28, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/48
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for transamidating a peptide substrate having a P.sub.1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S.sub.1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S.sub.1 ', S.sub.2 and/or S.sub.3 subsite to accommodate a specific peptide substrate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.