Dual chamber disposable reaction vessel for amplification reactions
US5989499A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 2, 1998 |
| Grant date | Nov 23, 1999 |
| Priority date | — |
| Expiry date | Apr 2, 2018 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0655
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction. A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an autlomated fashion without human intervention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.