Hapten assay by a competition-based method
US5989831A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 8, 1997 |
| Grant date | Nov 23, 1999 |
| Priority date | — |
| Expiry date | Jul 8, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method of assaying hapten in a sample, in which said sample is placed in contact with antibodies capable of recognizing hapten and with a predetermined quantity of a reagent capable of competing with said hapten-fixing antibodies. The reagent is a conjugate formed by a single-stranded fragment of nucleic acid with a compound capable of being recognized by said hapten-recognizing antibodies. The fixed or unfixed reactant is detected by antibodies capable of recognizing the nucleic acid fragment of said conjugate. Detection of said reactant by anti-fragment antibodies of said nucleic acid enhances, in particular, the sensitivity of the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.