Stable transformation of plant cells
US5990387A · kind A · utility
77Cited by
10References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 6, 1994 |
| Grant date | Nov 23, 1999 |
| Priority date | — |
| Expiry date | Oct 6, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8207
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.