Polymeric fluorophores enhanced by moieties providing a hydrophobic and conformationally restrictive microenvironment
US5994143A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 1, 1996 |
| Grant date | Nov 30, 1999 |
| Priority date | — |
| Expiry date | Feb 1, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/18
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Fluorescent conjugates suitable for use in flow cytometry and other biological applications. The fluorescent conjugates comprise an antibody having a polymeric dye bound thereto. The polymeric dye is preferably enhanced by a hydrophobic and conformationally restrictive moiety either bound thereto or in close association therewith. The hydrophobic and conformationally restrictive moiety is preferably derived from a cyclodextrin. The polymeric dye comprises a polymeric entity having signal-generating groups, such as aminostyryl pyridinium dye residues attached thereto. The fluorescent conjugates exhibit exceptional stability characteristics and avoid many of the problems of energy transfer, bio-conjugability, and solubility.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.