Mutated proteins encoded by a lentivirus mutated env gene, peptide fragments and expression vectors

US5994516A · kind A · utility

Assignee

• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR

Inventors

• Gianfranco Pancino · Paris, FR
• Pierre Sonigo · Paris, FR

Key dates

• Filing date: Dec 23, 1997
• Grant date: Nov 30, 1999
• Expiry date: Dec 23, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16122
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Mutated proteins coded by a mutated env gene of a lentivirus, particularly FIV, HIV or CAEV, peptide fragments contained in said mutated proteins and expression vectors expressing said mutaded proteins as well as the applications thereof are described. The peptide fragments are contained in the principal immunodominant domain (PID) of the transmembrane protein (TM) of lentiviruses and particularly of feline immunodeficiency virus (FIV), human immounodeficiency virus (HIV-1 and HIV-2), arthritis virus and caprine encephalitis virus (CAEV), and are capable of modifying the immunogenic properties of the PID domain and of the Env protein of the lentiviruses containing them. Such fragments have one of the following sequences: SEQ ID No1: CNQNQWLCK, SEQ ID No2: CNQNQFLCK, SEQ ID No3: CNQNQLWCK, SEQ ID No4: CNQNQPFCK, SEQ ID No5: CEHQHFFCK, SEQ ID No6: CSMGTFFCK, SEQ ID No7: CLTDSFFCK, SEQ ID No8: CELKNFFCK, SEQ ID No9: CRFAAFFCK, SEQ ID No10: ELGCGSKLICKIP, SEQ ID No11: IWGCNQNQFFCTTAVPWN, SEQ ID No12: IWGVAFRQVCTTAVPW.