Engraftment of immune-deficient mice with human cells
US5994617A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 17, 1994 |
| Grant date | Nov 30, 1999 |
| Priority date | — |
| Expiry date | Oct 17, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K67/0271
- WIPO fieldOther special machines
- WIPO sectorMechanical engineering
Abstract
A process for transplanting into an immunodeficient mouse, which is deficient in T-cells and B-cells, human cells to form a chimeric mouse is provided. The transplanted human cells proliferate and thereby permit in vivo study of the human cells. The human cells are isolated from a human tissue source. The process comprises: PA1 i) irradiating an immunodeficient mouse deficient in T-cells and B-cells with radiation to condition the mouse for transplant: PA1 ii) transplanting into the irradiated mouse, the isolated human cells; and PA1 iii) maintaining the mouse to proliferate the human cells in and permit the human cells to spread in the mouse, to provide thereby a chimeric mouse incorporating the human cells in appropriate murine tissue.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.