Production of chimeric bovine or porcine animals using cultured inner cell mass cells

US5994619A · kind A · utility

Assignee

• University of Massachusetts, A Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus · US

Inventors

• Steven Stice · Granby, US
• Jose Cibelli · Amherst, US
• James Robl · Granby, US
• Paul Golueke · Granby, US
• F. Abel Ponce de Leon · Amherst, US
• D. Joseph Jerry · Shutesbury, US

Key dates

• Filing date: Dec 16, 1996
• Grant date: Nov 30, 1999
• Expiry date: Dec 16, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2510/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substantially similarly to ICMs of undifferentiated developing embryos for prolonged culturing periods. Heterologous DNA is inserted into the subject CICM cells and cell lines so as produce transgenic CICM cell which are introduced into non-human fertilized embryos to produce transgenic chimeric embryos. The transgenic chimeric embryos are transferred into recipient females where they are permitted to develop into transgenic chimeric fetuses. Recipient females give birth to transgenic chimeric animals which are capable of transmitting the heterologous DNA to their progeny. Transgenic CICM cells are also used to produce cloned transgenic embryos, fetuses and offspring.