In planta method for the production of transgenic plants
US5994624A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1997 |
| Grant date | Nov 30, 1999 |
| Priority date | — |
| Expiry date | Oct 20, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8207
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for producing a transgenic plant in which a transforming agent such as a recombinant Agrobacterium or an isolated DNA molecule capable of conferring a desired phenotypic trait is injected into plant tissues using a needleless injection device. A preferred embodiment of the method provides the precise delivery of the transforming agent to floral tissues of a plant, allowing the direct insertion of the DNA of the transforming agent into germline cells of the plant that develop into seeds. This embodiment provides a more efficient method of transformation and subsequent regeneration of a transgenic plant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.