Method for performing polynucleotide separations using liquid chromatography
US5997742A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 18, 1998 |
| Grant date | Dec 7, 1999 |
| Priority date | — |
| Expiry date | May 18, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/528
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.