Glucose free, stable dry analytical element for quantitative analysis of creatine kinase MB isozyme
US5998158A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 22, 1998 |
| Grant date | Dec 7, 1999 |
| Priority date | — |
| Expiry date | Sep 22, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A dry analytical element for quantitatively analyzing creatine kinase MB isozyme is composed of a support and a creatine kinase MB isozyme-detecting agent layer. The detecting layer contains an antibody which specifically inhibits activity of M sub-unit of creatine kinase, creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase MB isozyme, a buffer compound which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound, said indicator composition having hexokinase, nicotinamide-adenine dinucleotide or its phosphate in an oxidized form, and glucose-6-phosphate dehydrogenase but not containing glucose in such amount as to give the spectroscopically detectable compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.