Methods for screening for antibiotics
US5998159A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 1998 |
| Grant date | Dec 7, 1999 |
| Priority date | — |
| Expiry date | Feb 10, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/18
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for identification of antibiotic agents which cause the accumulation of ppGpp in bacterial organisms involving a relA-independent pathway. The methods comprise screening assays in which test compounds are brought into contact with relA.sup.- test cells and observing the effect such compounds have on ppGpp levels in the test cells. The invention also provides genetically manipulated relA.sup.- test cells which contain a reporter gene the expression of which is sensitive to the level of ppGpp. The invention also encompasses agents identified by the screening assays, and uses of these agents in the treatment of infectious diseases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.