Method for in vitro protein synthesis
US5998163A · kind A · utility
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8Claims
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Key dates
| Filing date | Jan 13, 1999 |
| Grant date | Dec 7, 1999 |
| Priority date | — |
| Expiry date | Jan 13, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for performing coupled in vitro transcription and translation reactions is disclosed. In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.