Nitrilase from Rhodoccus rhodochrous for converting acrylonitrile directly to acrylic acid
US5998180A · kind A · utility
18Cited by
5References
15Claims
0Family size
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Key dates
| Filing date | Feb 24, 1998 |
| Grant date | Dec 7, 1999 |
| Priority date | — |
| Expiry date | Feb 24, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Nitrilase enzymes are provided which have Km at pH 7.0 for acrylonitrile of 500 .mu.M or below. The enzymes also have Ki at pH 7.0 for ammonium acrylate of at least 100 mM. In particular, the nitrilases have a value of the ratio of the said Ki to the said Km of at least 200. Particularly preferred nitrilases are obtainable from the microorganisms Rhodococcus rhodochrous NCIMB 40757 or NCIMB 40833. These nitrilases can be used in processes of converting acrylonitrile to ammonium acrylate in aqueous or vapor form and for detecting low levels of nitrile in aqueous or vapor form.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.