DNA polymerase having 3'-intrinsic editing activity

US6001566A · kind A · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Bruno Canard · Cambridge, US
• Gregor Sagner · Penzberg, DE

Key dates

• Filing date: May 31, 1996
• Grant date: Dec 14, 1999
• Expiry date: May 31, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/48
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention is directed to the use of RNA or DNA polymerases having 3'-intrinsic editing activity (3'-IEA) in the presence or absence of a deactivating agent to remove a protecting group from the 3'-position of oligo- polyribo- or deoxyribonnucleotides. The use is connected with the incorporation of dNTPs into DNA templates in order to determine the concentration and/or sequence of said templates. In particular the use is concerned with an improved non gel-based sequencing method.