Heteroduplex PCR cloning
US6001612A · kind A · utility
2Cited by
0References
16Claims
0Family size
Inventor
Key dates
| Filing date | Mar 10, 1999 |
| Grant date | Dec 14, 1999 |
| Priority date | — |
| Expiry date | Mar 10, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method of cloning a target nucleic acid sequence by performing two PCR reactions to generate two PCR products differing in terminal sequences. The PCR products thus obtained are denatured and annealed together to form a heteroduplex, which is ready for ligation and transformation of bacteria without any restriction digestion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.