Rhamnogalacturonase, corresponding DNA sequence, rhamnogalacturonase containing enzyme preparation and use of the enzyme preparation

US6001627A · kind A · utility

Assignee

• Novo Nordisk A/S · DK

Inventors

• Kurt Dorreich · Weil am Rhein, DE
• Henrik Dalb.o slashed.ge · Avenue Madeleine, DK
• Jan M. Mikkelsen · Copenhagen, DK
• Flemming M. Christensen · Hørsholm, DK
• Torben Halkier · Paseo Los Muchachos, DK

Key dates

• Filing date: Sep 11, 1995
• Grant date: Dec 14, 1999
• Expiry date: Sep 11, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01171
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to rhamnogalacturonases derived from a strain of Aspergillus japonicus which (a) has a pH-optimum between 6.5 and 7.0; (b) retains at least 80% of the maximal activity throughout the pH range of 5.5-12; (c) has a temperature optimum of about 40.degree. C.; and (d) retains at least 80% of the maximal activity throughout the temperature range of 20-60.degree. C. The present invention relates to rhamnogalacturonases derived from a strain of Aspergillus aculeatus which (a) has a pH-optimum of about 5.0; (b) retains at least 80% of the maximal activity throughout the pH range of 3-6.5; (c) has a temperature optimum of about 40.degree. C.; and (d) retains at least 80% of the maximal activity throughout the temperature range of 5-50.degree. C.