Debranching enzymes and DNA sequences coding them, suitable for changing the degree of branching of amylopectin starch in plants

US6001628A · kind A · utility

Assignee

• Hoechst Schering Agrevo GmbH · DE

Inventors

• Jens Kossmann · Golm, DE
• Michael Emmermann · Michendorf, DE
• Ivar Virgin · Stockholm, SE

Key dates

• Filing date: Apr 18, 1996
• Grant date: Dec 14, 1999
• Expiry date: Apr 18, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/2451
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

DNA sequences are described which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants code new proteins with the enzymatic activity of debranching enzymes which in transgenic plants reduce the degree of branching of amylopectin starch and DNA sequences which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants prevent the synthesis of proteins with the enzymatic activity of debranching enzymes, which in the transgenic plants increases the degree of branching of amylopectin starch, as well as plasmids on which these DNA sequences are localized, which can be introduced into plant cells and plants. Also described is a process for the production of plants changed by genetic engineering whose amylopectin starch is modified, and the modified starch obtainable from these plants.