Method of separating albumin from serum by ion-exchange chromatography with membrane adsorbers

US6001974A · kind A · utility

Assignee

• Sartorius AG · DE

Inventors

• Wolfgang Demmer · Göttingen, DE
• Heinrich Klaus Gebauer · Jülich, DE
• Maria-Regina Kula · Jülich, DE
• Dietmar Nussbaumer · Göttingen, DE
• Jorg Thoemmes · Bonn, DE

Key dates

• Filing date: Mar 10, 1998
• Grant date: Dec 14, 1999
• Expiry date: Mar 10, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/765
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of separating albumin from serum by ion exchange chromatography with membrane adsorbers characterized by high productivity and yields of high purity albumin. The separation of the albumin is carried out on highly basic anion exchange membranes and on highly acidic cation exchange membranes. The albumin fraction can be eluted from the anion exchange membrane such that it can be fed directly to the cation exchange membrane without any special conditioning and the albumin can be extracted therefrom as an end product.