Method for cloning and producing the AvaI restriction endonuclease in E. coli and purification of the recombinant AvaI restriction endonuclease
US6004793A · kind A · utility
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5Claims
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Key dates
| Filing date | Aug 18, 1998 |
| Grant date | Dec 21, 1999 |
| Priority date | — |
| Expiry date | Aug 18, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant restriction endonuclease from Anabaena variabilis is provided, as well as the isolated gene which encodes it and methods for the production of the recombinant AvaI restriction endonuclease. An isolated gene encoding a modification methylase from A. variabilis is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.