Genes encoding HSV1-TK containing a deletion of 5' cryptic promoter sites, vectors and cell lines
US6004803A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 1997 |
| Grant date | Dec 21, 1999 |
| Priority date | — |
| Expiry date | Apr 11, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16043
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A recombinant vector comprising a gene encoding a Herpes Simplex Virus Type 1 thymidine kinase (HSV1-TK) comprising a deletion in the 5' sequence upstream of the second ATG initiation codon of the complete HSV1-TK sufficient to inhibit transcription initiation from a cryptic promoter site contained within the 5' sequence, wherein the gene is under the control of a promoter is disclosed for use as a suicide gene both in vitro and in vivo. The HSV1-TK produced by the strong expression of the modified gene is toxic to cells in the presence of nucleoside analogs, whereas weak expression of the gene is not toxic to cells. The deletion can comprise all or part of the first initiation codon and the vector can be a retrovirus. As also disclosed are cells transduced with the vector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.