Repair-mediated process for amplifying and detecting nucleic acid sequences
US6004826A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 27, 1993 |
| Grant date | Dec 21, 1999 |
| Priority date | — |
| Expiry date | Oct 27, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/703
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a process for amplifying and detecting any desired specific nucleic acid sequence that exists in a nucleic acid or mixture thereof. The process comprises treating single strand RNA or separated complementary strands of DNA target with a molar excess of oligonucleotide complement pairs in which these oligonucleotide complement pairs have sequences complementary to the target, under hybridizing conditions. In one embodiment, the oligonucleotide complement pairs may have a gap of one or more bases which may be repaired (filled) by enzymes. The oligonucleotide complement pairs are joined together, forming joined, oligonucleotide product. The target/joined product hybrid nucleic acids are then denatured to single strands again, at which point both the target and the joined products can form hybrids with new oligonucleotide complement pairs. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.