Simmondsin concentrate from jojoba

US6007823A · kind A · utility

Assignee

• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• Thomas P. Abbott · Peoria, US
• Brian J. Plattner · Reynoldsburg, US
• Hal Purcell · Avila Beach, US

Key dates

• Filing date: Jun 2, 1998
• Grant date: Dec 28, 1999
• Expiry date: Jun 2, 2018

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K31/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A process for the isolation of simmondsins from jojoba meal is disclosed. Simmondsins are first extracted from defatted jojoba meal with water. After separation from the meal, this water extract is subsequently dried, leaving the simmondsins in the resulting solid. The simmondsins may then be preferentially extracted from the dried water extract by contact with a first ethanolic solvent, forming a first solvent fraction containing simmondsins therein. The first solvent fraction, which is a substantially protein-free concentrate of simmondsins, may be separated from the solid phase, and the ethanolic solvent may then be readily removed, for example, by drying. Residual simmondsins remaining in the meal following extraction with the first ethanolic solvent may also be recovered by a second extraction with ethanolic solvent. Optionally, simmondsin and its analogs, simmondsin ferulate, demethylsimmondsin, and didemethyl simmondsin, may be separated in substantially pure form from the extracts by reverse-phase HPLC.