Monoclonal antibodies specific for PHF-tau, hybridomas secreting them, antigen recognition by these antibodies and their applications

US6008024A · kind A · utility

Assignee

• Innogenetics N.V. · BE

Inventors

• Marc Vandermeeren · Geel, BE
• Eugeen Vanmechelen · Zevergem, BE
• Andre Van De Voorde · Lokeren, BE

Key dates

• Filing date: Jun 20, 1996
• Grant date: Dec 28, 1999
• Expiry date: Jun 20, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/4711
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Monoclonal antibody AT180 secreted by the hybridoma deposited at ECACC on Dec. 22, 1992 under No. 92122204, and monoclonal antibody AT270 secreted by the hybridoma deposited at ECACC on Jul. 7, 1993 under 93070774, each of which forms an immunological complex with a phosphorylated epitope of an antigen belonging to abnormally phosphorylated tau protein (PHF-tau) residing in the region spanning positions 143-254 with the following amino acid sequence: 143 150 NH.sub.2 - Lys Gly Ala Asp Gly Lys Thr Lys Ile - 160 Ala Thr Pro Arg Gly Ala Ala Pro Pro Gly - 170 Gln Lys Gly Gln Ala Asn Ala Thr Arg Ile - 180 Pro Ala Lys Thr Pro Pro Ala Pro Lys Thr - 190 Pro Pro Ser Ser Gly Glu Pro Pro Lys Ser - 200 Gly Asp Arg Ser Gly Tyr Ser Ser Pro Gly - 210 Ser Pro Gly Thr Pro Gly Ser Arg Ser Arg - 220 Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg - 230 Glu Pro Lys Lys Val Ala Val Val Arg Thr - 240 Pro Pro Lys Ser Pro Ser Ser Ala Lys Ser - 250 Arg Leu Gln Thr Ala Pro Val Pro Met Pro - Asp Leu Lys COOH with each monoclonal antibody specifically detecting abnormally phosphorylated tau protein (PHF-tau) in cerebrospinal fluid (CSF).