Recombinant allergen, fragments thereof, corresponding recombinant DNA molecules, vectors and hosts containing the DNA molecules

US6008340A · kind A · utility

Assignee

• Pharmacia & UpJohn AB · SE

Inventors

• Tanja Ball · Gleisdorf, AT
• Susanne Vrtala · Wien, AT
• Wolfgang Sperr · Wien, AT
• Peter Valent · Wien, AT
• Markus Susani · Salzburg, AT
• Dietrich Kraft · Wien, AT
• Rudolf Valenta · Theresienfeld, AT
• Sylvia Laffer · Wien, AT

Key dates

• Filing date: Jan 14, 1997
• Grant date: Dec 28, 1999
• Expiry date: Jan 14, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A recombinant DNA molecule comprising a nucleocide sequence (I) which codes for a polypeptide displaying the antigenicity of one, two or more of the Phl p I epitope clones (28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114), with the amino acid sequences defined in SEQ ID NOS: 5, 7, 9 and 12-28, and preferably being derived from grasses or monocotyledonic plants, or a nucleotide sequence (II) which hybridizes with such a nucleotide sequence (I) under conditions of high stringency. Polypeptides displaying the antigenicity of one, two or more of the Phl p I epitope clones (28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114). Recombinant expression vectors containing the recombinant molecule and host cells transformed with the vector. Diagnostic methods based on utilizing the polypeptides in immunoassays for humoral antibodies and cellular reactions.