Orthoester reagents for use as protecting groups in oligonucleotide synthesis
US6008400A · kind A · utility
94Cited by
4References
15Claims
0Family size
Inventors
Key dates
| Filing date | Dec 19, 1997 |
| Grant date | Dec 28, 1999 |
| Priority date | — |
| Expiry date | Dec 19, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Phosphoramidite oligonucleotide synthesis is facilitated by the use of fluoride-labile 5' silyl protecting groups. RNA synthesis is improved by the use of 2 orthoester protecting groups. Reactions are conducted on a solid phase support and acidic deprotection conditions are avoided, as is the necessity of oxidizing the phosphite linkage between each coupling reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.