Method of screening for attenuating viruses
US6010894A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 13, 1997 |
| Grant date | Jan 4, 2000 |
| Priority date | — |
| Expiry date | Jun 13, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method of selecting virus vaccine candidates by selecting virus variants that do not bind to brain membrane receptor preparations, comprising the steps of: (a) preparing a brain membrane receptor preparation; (b) mixing an amount of a virus of interest with an amount of said membrane receptor preparation containing excess membrane receptors to form a virus-membrane receptor preparation suspension; (c) centrifuging said suspension to form a supernatant; (d) determining residual virus infectivity in said supernatant; and (e) isolating individual membrane receptor preparation binding-resistant virus variants which are useful as virus vaccine candidates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.