Method for reducing the linear dimension necessary for high resolution electrophoretic separation
US6013166A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 28, 1994 |
| Grant date | Jan 11, 2000 |
| Priority date | — |
| Expiry date | Apr 28, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an electrophoretic method to effect differential net migration, the extent of said migration being dependent on molecular size, of electrically charged macromolecules through a gel support in a single dimension, which method comprises subjecting electrically charged macromolecules applied to a gel support to an electric field oriented along a single axis within the gel for a time period sufficient to effect migration in the direction of the oriented field and form a separation pattern in order of the respective molecular weights of the macromolecules in a distance of about 0.5 to about 20 millimeters, said gel support comprising about 3 to about 40 percent polyacrylamide, said electric field being applied in an amount of about 5 to about 100 volts per millimeter of gel support along the axis length, and said gel support having a width perpendicular to said axis of about 0.1 to 1.5 millimeters.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.