Direct quantitation of low copy number RNA
US6013442A · kind A · utility
18Cited by
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7Claims
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Key dates
| Filing date | Aug 5, 1997 |
| Grant date | Jan 11, 2000 |
| Priority date | — |
| Expiry date | Aug 5, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A direct quantitation of RNA contained in a sample is obtained by capillary electrophoresis of the RNA hybridized to a DNA probe of complementary sequence stabilized by the combination of a fluorophore terminally conjugated to the DNA probe and a dye intercalating the RNA-DNA hybrid so formed. The RNA is quantified by measuring the total fluorescence emitted by the electrophoresed hybrid upon excitation by a laser generated light beam.
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