Massively parallel signature sequencing by ligation of encoded adaptors
US6013445A · kind A · utility
452Cited by
23References
29Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 7, 1997 |
| Grant date | Jan 11, 2000 |
| Priority date | — |
| Expiry date | Oct 7, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/149
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method of nucleic acid sequence analysis based on the ligation of one or more sets of encoded adaptors to the terminus of a target polynucleotide. Encoded adaptors whose protruding strands form perfectly matched duplexes with the complementary protruding strands of the target polynucleotide are ligated, and the identity of the nucleotides in the protruding strands is determined by an oligonucleotide tag carried by the encoded adaptor. Such determination, or "decoding" is carried out by specifically hybridizing a labeled tag complement to its corresponding tag on the ligated adaptor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.